R&D Systems™ Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein

Description

Recombinant Protein

Measured by its binding ability in a functional ELISA. When Recombinant Canine PD-1 Fc Chimera (Catalog # 10553-PD ) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED50 of 0.30-4.50 μg/mL.

Specifications

• Accession Number: NP_001278901.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 29126
• Molecular Weight (g/mol): M.W.-observed: 60-80 kDa, under reducing conditions, M.W.-theroretical: 51 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived canine PD-L1/B7-H1 protein Canine PD-L1/B7-H1 (Phe19-Arg236) (N-terminus) Accession # NP_001278901.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Avelumab, B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-L1