R&D Systems™ Recombinant Cyno u-Plasminogen Activator (uPA) Protein

Description

His-tag

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: XP_05565501.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl2.
• Gene ID (Entrez): 5328
• Molecular Weight (g/mol): M.W.-observed: 32-41 kDa (short form) and 48-59 kDa (long form), non-reducing conditions, M.W.-theroretical: 18 kDa (long A chain), 3 kDa (short A chain), 30 kDa (B chain)
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey u-Plasminogen Activator (uPA)/Urokinase protein Ser21-Leu430 with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: ATF, EC 3.4.21, EC 3.4.21.73, plasminogen activator, urokinase, PLAU, uPA, u-PA, uPlasminogen Activator, U-plasminogen activator, urinary, Urokinase, urokinase-type plasminogen activator
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: u-Plasminogen Activator/Urokinase