R&D Systems™ Recombinant Cynomolgus 5'-Nucleotidase/CD73 His Protein

Description

CD73, known as ecto-5'-Nucleotidase, converts extracellular nucleoside 5' monophosphates to nucleosides, with AMP as its preferred substrate

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >6500 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: XP_005552488.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Gene ID (Entrez): 4907
• Molecular Weight (g/mol): M.W. (Observed): 57-75 kDa; M.W. (theoretical): 59 kDa
• Purification Method: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey 5 '-Nucleotidase/CD73 protein Trp27-Lys547 with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: 5 '-Nucleotidase/CD73