R&D Systems™ Recombinant Cynomolgus LIFR alpha Fc Chimera Protein

Description

Carrier Free Recombinant Protein

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 0.7-7 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.

Specifications

• Accession Number: XP_005556818.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3977
• Molecular Weight (g/mol): M.W.-observed: 133-149 kDa, under reducing conditions, M.W.-theoretical: 116 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey LIFR alpha protein Cynomolgus Monkey LIFR alpha (Gln45-Ser833) (N-terminus) Accession # XP_005556818.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD118, CD118 antigen, FLJ98106, FLJ99923, leukemia inhibitory factor receptor, leukemia inhibitory factor receptor alpha, LIF R alpha, LIF R beta, LIF receptor, LIFR, LIF-R, LIFRa, SJS2, STWS, SWS
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: LIFR alpha