R&D Systems™ Recombinant Cynomolgus Monkey IL-27 His-tag Protein

Catalog No.	Quantity
10-307-IL025	25 μg

Catalog No. 10-307-IL025 Supplier R&D Systems™ Supplier No. 10307IL025

Description

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus.

Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.75-6.0 ng/mL.

Specifications

• Accession Number: XP_005587614.1 (EBI-3) & XP_028697194.1 (IL-27a)
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
• Gene ID (Entrez): 246778
• Molecular Weight (g/mol): Observed: 54-65 kDa, under reducing conditions; Theroretical: 50 kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-27 protein Cynomolgus Monkey EBI-3 (Arg36-Lys244) Accession # XP_005587614.1 GSGSSRGGSGSGGSGGGGSKL

  Cynomolgus Monkey IL-27 A *Unique sequence

  6-His tag N-terminus C-terminus * Unique Sequence: This gene was isolated from a cynomolgus monkey cDNA library. The closest match to the obtained sequence is a genomic prediction for rhesus monkey, Accession XP_028697194.1 (Phe42-Pro254, A170T).
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: IL-27