R&D Systems™ Recombinant Cynomolgus Monkey SIRP alpha/CD172a Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey SIRP alpha/CD172a is coated at 2 μg/mL, 100 μL/well, Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD ) binds with an ED50 of 10-60 ng/mL.

Specifications

• Accession Number: NP_001271679
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 140885
• Molecular Weight (g/mol): M.W. (Observed): 52-67 kDa, reducing conditions; M.W. (theoretical): 38 kDa
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived cynomolgus monkey SIRP alpha/CD172a protein Gly27 & Glu31-Arg369, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Bit, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MFRtyrosine phosphatase SHP substrate 1, MYD1, MYD-1, MyD-1 antigen, P84, protein tyrosine phosphatase, non-receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, signal-regulatory protein alpha, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRPA, SIRPalpha, SIRP-ALPHA-1, Sirp-alpha-2, SIRPalpha2, Sirp-alpha-3, SIRPtyrosine-protein phosphatase non-receptor type substrate 1
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Cynomolgus Monkey
• Reconstitution: Reconstitute at 1 mg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: SIRP alpha/CD172a