R&D Systems™ Recombinant Cynomolgus Monkey Tie-2 Fc Chimera Protein

Description

Measured by its binding ability in a functional ELISA.

When Recombinant Cynomolgus Monkey Tie-2 Fc Chimera (Catalog # 10376-T2) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN ) binds with an ED50 of 1.5-15 ng/mL.

Specifications

• Accession Number: XP_005581645.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 7010
• Molecular Weight (g/mol): Observed: 118-132 kDa, under reducing conditions; Theroretical: 107 kDa
• Quantity: 100 μg
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey Tie-2 protein Cynomolgus Monkey Tie-2 (Ala23-Lys745) Accession # XP_005581645.1(N-terminus) IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 1 mg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: Tie-2