R&D Systems™ Recombinant Cynomolgus PD-1 His-tag Protein

Catalog No.	Quantity
85-09P-D050	50 μg

Catalog No. 85-09P-D050 Supplier R&D Systems™ Supplier No. 8509PD050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL ) is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey PD-1, in the presence of Ms x Poly His, that produces 50% optimal binding response is typically 0.3-1.5 μg/mL.

Specifications

• Accession Number: NP_001271065.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in sterile PBS.
• Gene ID (Entrez): 5133
• Molecular Weight (g/mol): M.W. (Observed): 29-43 kDa, reducing conditions; M.W. (theoretical): 17 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey PD-1 protein Leu25-Gln167, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
• Common Name: PD-1
• Gene Symbol: PDCD1
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2/B7-DC Fc Chimera is coated at 0.5μg/mL (100μL/well), the concentration of Recombinant Cynomolgus Monkey PD-1, in the presence of Ms x Poly His, that produces 50% optimal binding response is typically 0.3-1.5μg/mL.
• Conjugate: Unconjugated
• Species: Cynomolgus Monkey
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Expression System: Human embryonic kidney cell, HEK293-derived Leu25-Gln167, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-1