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R&D Systems™ Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein

Catalog No. 10960NK050
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10960NK050 50 μg
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Recombinant Protein

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera (Catalog # 10960-NK) is immobilized at 2 μg/mL (100 μL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (Catalog # AVI1300 ) binds with an ED50 of 25.0-200 ng/mL.

Specifications

Accession Number P61252.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 22914
Molecular Weight (g/mol) M.W.-observed: 45-65 kDa, under reducing conditions., M.W.-theroretical: 43 kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line, CHO-derived NKG2D/CD314 protein MD Human IgG1 (Pro100-Lys330) IEGR Cynomologus Monkey NKG2D (Trp74-Val216) (N-terminus) Accession # P61252.1 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias CD314, CD314 antigen, D12S2489E, FLJ17759, FLJ75772, Killer cell lectin-like receptor subfamily K member 1, killer cell lectin-like receptor subfamily K, member 1, KLR, KLRK1, NK cell receptor D, NKG2-D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
Conjugate Unconjugated
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein NKG2D/CD314
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