R&D Systems™ Recombinant Cynomolgus Siglec-2/CD22 Fc Chimera Protein

Catalog No.	Quantity
10-031-SL050	50 μg

Catalog No. 10-031-SL050 Supplier R&D Systems™ Supplier No. 10031SL050

Description

The Recombinant Cynomolgus Siglec-2/CD22 Fc Chimera Protein is derived from HEK293

• Siglecs are sialic acid specific I-type lectins that are characterized by an extracellular domain (ECD) with an N-terminal Ig-like V-type domain followed by varying numbers of Ig-like C2-type domains.
• Siglec-2, also known as B cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells.
• In humans, two distinct Siglec-2 cDNAs that arise from differential RNA processing of the same gene have been isolated.
• Within the ECD, cynomolgus Siglec-2 shares 84%, 55%, and 56% aa sequence identity with human, mouse, and rat Siglec-2, respectively.
• Siglec-2 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells.
• Consistent with a single ligand-binding region, the first two N-terminal Ig-like domains mediated CD22 adhesion with lymphocytes, neutrophils, monocytes, and erythrocytes.
• Besides its role as an adhesion molecule, Siglec-2 is a co-receptor that physically interacts with B cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation.
• It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs.
• Phosphorylated Siglec-2 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling.

Specifications

• Accession Number: EHH59463
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 933
• Molecular Weight (g/mol): 102 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 50 μg
• Source:

  Human embryonic kidney cell, HEK293-derived cynomolgus monkey Siglec-2/CD22 protein

  Cynomolgus Monkey Siglec-2/CD22 (Asp20-Arg687) Accession # EHH59463IEGRMDHuman IgG1 (Pro100-Lys330)N-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 250 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose