R&D Systems™ Recombinant E. coli Methionine Aminopeptidase/METAP

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017 ). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE ). The specific activity is >0.5 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com .

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017 ). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE ). The specific activity is >0.5 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com .

Specifications

• Accession Number: NP_414710
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Gene ID (Entrez): 947882
• Molecular Weight (g/mol): M.W. (Observed): 36 kDa, reducing conditions; M.W. (theoretical): 31 kDa
• Name: Methionine Aminopeptidase
• Quantity: 20 μg
• Source: E. coli-derived e. coli Methionine Aminopeptidase protein Ala2-Glu264, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: map
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Methionine Aminopeptidase