R&D Systems™ Recombinant E. faecalis O-Glycosidase Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to hydrolyze p-Nitrophenyl galacto-N-bioside. The specific activity is >12,500 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q833X5
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 128 kDa, reducing conditions; M.W. (theoretical): 145 kDa
• Quantity: 20 μg
• Source: E. coli-derived e. faecalis O-Glycosidase protein Glu29-Lys1324, with N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Common Name: O-Glycosidase
• Biological Activity: Measured by its ability to hydrolyze p-Nitrophenyl galacto-N-bioside. The specific activity is >12,500 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: E. faecalis
• Recombinant: Recombinant
• Expression System: E. coli-derived Glu29-Lys1324, with N-terminal Met and 6-His tag
• Purity or Quality Grade: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: O-Glycosidase