R&D Systems™ Recombinant Equine PDGF-BB Protein

Catalog No.	Quantity
8585BB010	10 μg

Catalog No. 8585BB010 Supplier R&D Systems™ Supplier No. 8585BB010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Equine PDGF-BB Protein is derived from E. coli. The Recombinant Equine PDGF-BB Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: XP_005606703
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 5155
• Molecular Weight (g/mol): Observed M.W.: 12kDa, reducing conditions, Theoretical M.W.: 12kDa
• Quantity: 10 μg
• Source: E. coli-derived equine PDGF-BB protein Ser51-Thr159
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: PDGF-BB
• Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 5-30ng/mL.
• Conjugate: Unconjugated
• Species: Equine
• Recombinant: Recombinant
• Expression System: E. coli-derived Ser51-Thr159
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining