R&D Systems™ Recombinant Escherichia coli Metalloprotease/StcE His, CF

Description

His-tag

Measured by its ability to cleave Recombinant CD45 Protein at specific O-glycan sites. One μg of Recombinant E. coli StcE will cleave >60% of Recombinant CD45 Protein with labeled O-glycan (Catalog # 1430-CD ), as measured under the described conditions.

Specifications

• Accession Number: O82882.2
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 86-95 & 53-58 kDa, under reducing conditions., M.W. (Theoretical): 97 kDa & 54 kDa
• Quantity: 50 μg
• Source: E. coli-derived e. coli StcE protein Ala36-Lys898 with a N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Purity or Quality Grade: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: StcE