R&D Systems™ Recombinant F. meningosepticum Endo F1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to deglycosylate ribonuclease B under native conditions. The DC50 is < 5 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 μg of RNase B in 30 minutes at 37 °C. . Use of Recombinant F. meningosepticum Endo F1 in the delycoslyation of other substrates may require alternative conditions for optimal performance.

Specifications

• Accession Number: P36911
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 35 kDa, under reducing conditions; M.W. (theoretical): 35 kDa
• Name: Endo-beta-N-acetylglucosaminidase F1/Endo F1
• Quantity: 50 μg
• Source: E. coli-derived f. meningosepticum Endo-beta-N-acetylglucosaminidase F1/Endo F1 protein Arg31-Trp339, with an N-terminal Met and 6-His tag Accession # P36911
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: Endo-beta-N-acetylglucosaminidase F1/Endo F1