R&D Systems™ Recombinant F. meningosepticum Endo F3 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to deglycosylate TSLP under native conditions. The DC50 is <12 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 μg of recombinant mouse TSLP in 30 minutes at 37 °C. . Use of Recombinant F. meningosepticum Endo-beta-N-acetylglucosaminidase F3/Endo F3 in the delycoslyation of other substrates may require alternative conditions for optimal performance.

Specifications

• Accession Number: P36913
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
• Molecular Weight (g/mol): M.W. (Observed): 30 kDa, reducing conditions; M.W. (theoretical): 32 kDa
• Name: Endo-beta-N-acetylglucosaminidase F3/Endo F3
• Quantity: 10 μg
• Source: E. coli-derived f. meningosepticum Endo-beta-N-acetylglucosaminidase F3/Endo F3 protein Ala44-Asn329, with an N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Endo-beta-N-acetylglucosaminidase F3/Endo F3