R&D Systems™ Recombinant F. meningosepticum PNGase F' (CystatinA Tag)

Catalog No.	Quantity
7695GHP020	20 μg

Catalog No. 7695GHP020 Supplier R&D Systems™ Supplier No. 7695GHP020

Description

rFm PNGase F is also available without the CystatinA Tag!

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 μg) is deglycosylated by 30 ng of Recombinant F. meningosepticum PNGase F' within 30 minutes, as measured under the described conditions.

Specifications

• Accession Number: AAA85323 (PNGase F') & P01040 (Cystatin A)
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
• Molecular Weight (g/mol): M.W. (Observed): 44-48 kDa, reducing conditions; M.W. (theoretical): 48 kDa
• Name: PNGase F ' (Cystatin A Tag)
• Quantity: 20 μg
• Source: E. coli-derived f. meningosepticum PNGase F protein Met 6-His tag Cystatin A (Met1-Phe98) (N-terminus) Accession # P01040 Linker PNGase F (Ala41-Asn354) (N-terminus) Accession # AAA85323 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane)
• Protein: PNGase F