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R&D Systems™ Recombinant F. tularensis Cpf1 Protein
SDP

Catalog No. 10343C1100 Shop All R&D Systems Products
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100 μg
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10-343-C1100 100 μg
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Catalog No. 10-343-C1100 Supplier R&D Systems™ Supplier No. 10343C1100
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Description

Measured by its ability to cleave a targeted DNA substrate.

rF. tularensis Cpf1 achieves >80% substrate cleavage, as measured under the described conditions.

Specifications

Accession Number WP_003034647.1
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, Glycerol and TCEP.
Molecular Weight (g/mol) Observed: 110-135 kDa, under reducing conditions
Quantity 100 μg
Source E. coli-derived f. tularensis Cpf1 protein APKKKRKVGIHGVPAA F. tularensis Cpf1 (Ser2-Asn1300) Accession # WP_003034647.1(N-terminus) KRPAATKKAGQAKKKKGG HHHHHH (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Protein CRISPR-Cpf1
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