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R&D Systems™ Recombinant Human 4-1BB Ligand/TNFSF9 Protein
GSA_VA

Catalog No. 22954L025
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG and with Trehalose.
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG with BSA as a carrier protein.
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Catalog No. Formulation
22954L025 Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG with BSA as a carrier protein.
22954L025CF Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG and with Trehalose.
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Catalog No. 22954L025 Supplier R&D Systems™ Supplier No. 22954L025
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Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 838-4B ) is Immobilized at 10 ng/mL (100 μL/well), the concentration of rh4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.

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Specifications

Accession Number P41273.1
For Use With (Application) Binding Activity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG with BSA as a carrier protein.
Gene ID (Entrez) 8744
Molecular Weight (g/mol) M.W. (theoretical): 20.4 kDa
Quantity 25 μg
Source E. coli-derived human 4-1 BB Ligand/TNFSF9 protein Arg71-Glu254, with an N-terminal Met and a 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 3 months, 2°C to 8°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Symbol TNFSF9
Product Type 4-1BB Ligand/TNFSF9
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein 4-1 BB Ligand/TNFSF9
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