R&D Systems™ Recombinant Human 4-1BB Ligand/TNFSF9 Protein

Catalog No.	Formulation
22954L025CF	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG and with Trehalose.
22954L025	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG with BSA as a carrier protein.

Catalog No. 22954L025CF Supplier R&D Systems™ Supplier No. 22954L025/CF

Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 838-4B ) is Immobilized at 10 ng/mL (100 μL/well), the concentration of rh4-1BB Ligand that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.

Specifications

• Accession Number: P41273.1
• For Use With (Application): Binding Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG and with Trehalose.
• Gene ID (Entrez): 8744
• Molecular Weight (g/mol): M.W. (theoretical): 20.4 kDa
• Quantity: 25 μg
• Source: E. coli-derived human 4-1 BB Ligand/TNFSF9 protein Arg71-Glu254, with an N-terminal Met and a 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 3 months, 2°C to 8°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TNFSF9
• Product Type: 4-1BB Ligand/TNFSF9
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: 4-1 BB Ligand/TNFSF9