R&D Systems™ Recombinant Human 5'-Nucleotidase/CD73 Fc Avi-tag, CF

Catalog No.	Quantity
AVI11377025	25 μg

Catalog No. AVI11377025 Supplier R&D Systems™ Supplier No. AVI11377025

Description

Fc Chimera

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >5500 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human 5'-Nucleotidase/CD73 Fc Chimera Avi-tag (Catalog # AVI11377) binds Human/Equine 5'-Nucleotidase/CD73 Antibody (Catalog # MAB5795 ) with an ED50 of 3.00-30.0 ng/mL.

Specifications

• Accession Number: AAH65937.1
• For Use With (Application): Enzyme Activity, Binding Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Gene ID (Entrez): 4907
• Molecular Weight (g/mol): M.W. (Observed): 88-98 kDa, under reducing conditions, M.W. (Theroretical): 86 kDa
• Quantity: 25 μg
• Source: Human embryonic kidney cell, HEK293-derived human 5'-Nucleotidase/CD73 protein Human 5'-Nucleotidase/CD73 (Trp27-Lys547) (N-terminus) Accession # AAH65937.1 GGIEGRMD Human IgG1 (Pro100-Lys330) Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU per 1 μg of the protein by the LAL method.
• Gene Alias: 5' nucleotidase (CD73), 5'-NT, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, EC 3.1.3.5, ecto-5'-nucleotidase, eN, eNT, NT, NT55'-nucleotidase, NTE, Purine 5-Prime-Nucleotidase
• Conjugate: Biotin
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: 5'-Nucleotidase/CD73