R&D Systems™ Recombinant Human 5'-Nucleotidase/CD73 Protein

Catalog No.	Quantity
57-95E-N500	500 μg

Catalog No. 57-95E-N500 Supplier R&D Systems™ Supplier No. 5795EN500

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Order Info

Ships at ambient temperature.

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Specifications

• Accession Number: AAH65937
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Gene ID (Entrez): 4907
• Molecular Weight (g/mol): M.W. (Observed): 61-62 kDa, reducing conditions; M.W. (theoretical): 59 kDa
• Quantity: 500 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human 5 '-Nucleotidase/CD73 protein Trp27-Lys547, with a C-terminal 6-His tag, Trp27-Lys547
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: NT5E
• Biological Activity: >15,000pmol/min/μg
• Product Type: Recombinant protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: 5 '-Nucleotidase/CD73

For Research Use Only.