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R&D Systems™ Recombinant Human 5'-Nucleotidase/CD73 Protein
GSA_VA

Catalog No. 5795EN500
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Quantity:
500 μg
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Order Info

Ships at ambient temperature.

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Specifications

Accession Number AAH65937
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Gene ID (Entrez) 4907
Molecular Weight (g/mol) M.W. (Observed): 61-62 kDa, reducing conditions; M.W. (theoretical): 59 kDa
Quantity 500 μg
Source Chinese Hamster Ovary cell line, CHO-derived human 5 '-Nucleotidase/CD73 protein Trp27-Lys547, with a C-terminal 6-His tag, Trp27-Lys547
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Regulatory Status RUO
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Symbol NT5E
Biological Activity >15,000pmol/min/μg
Product Type Recombinant protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Purity or Quality Grade >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein 5 '-Nucleotidase/CD73
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For Research Use Only.

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