R&D Systems™ Recombinant Human ACE-2 His-tag Biotinylated Protein

Catalog No.	Quantity
BT933020	20 μg

Catalog No. BT933020 Supplier R&D Systems™ Supplier No. BT933020

Description

Biotinylated via Amines

Biotinylated Recombinant Human ACE-2 His-tag (Catalog # BT933) binds Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV ) in a functional ELISA. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >600 pmol/min/ μg, as measured under the described conditions.

Specifications

• Accession Number: Q9BYF1.2
• For Use With (Application): Bioactivity, Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
• Gene ID (Entrez): 59272
• Molecular Weight (g/mol): M.W. Observed: 100-110 kDa, under reducing conditions, M.W. Theoretical: 85 kDa
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human ACE-2 protein Gln18-Ser740 with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: ACE2, ACEH, ACEHangiotensin I converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15
• Conjugate: Biotin
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: ACE-2