R&D Systems™ Recombinant Human ACE-2 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV ) is immobilized at 0.2 μg/mL (100 μL/well), Recombinant Human ACE-2 (Catalog # 933-ZN) binds with an ED50 of 1.00-12.0 ng/mL. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >800 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q9BYF1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
• Gene ID (Entrez): 59272
• Molecular Weight (g/mol): M.W. (Observed): 101-111 kDa, reducing conditions; M.W. (theoretical): 85 kDa
• Name: ACE-2
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human ACE-2 protein Gln18-Ser740, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Structural Form: Recombinant Human ACE-2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: ACE2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Activity: Measured by its binding ability in a functional ELISA with Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >800 pmol/min/μg, as measured under the described conditions.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: ACE-2