R&D Systems™ Recombinant Human Active Heparanase/HPSE Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to release biotinylated heparan sulfate from Recombinant Human Syndecan-4 (Catalog # 2918-SD ). 20 ng of Recombinant Human Active Heparanase/HPSE digestion will result in >50% of OD reduction compared with the Negative Control, as measured under the described conditions.

Specifications

• Accession Number: AF144325
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and E64.
• Gene ID (Entrez): 10855
• Molecular Weight (g/mol): M.W. (Observed): 50-60 kDa & 7-9 kDa, reducing conditions; M.W. (theoretical): 43 kDa & 9 kDa
• Name: Active Heparanase/HPSE
• Quantity: 5 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Heparanase/HPSE protein Gln36-Ile543 with N-terminal 6-His tag The linker peptide from Ser110-Gln157 is removed by protease treatment
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: HPSE
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Heparanase/HPSE