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R&D Systems™ Recombinant Human Active Heparanase/HPSE Protein

Catalog No. 7570GH005
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5ug; Carrier Free
This item is not returnable. View return policy
This item is not returnable. View return policy

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to release biotinylated heparan sulfate from Recombinant Human Syndecan-4 (Catalog # 2918-SD ). 20 ng of Recombinant Human Active Heparanase/HPSE digestion will result in >50% of OD reduction compared with the Negative Control, as measured under the described conditions.

Specifications

Accession Number AF144325
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and E64.
Gene ID (Entrez) 10855
Molecular Weight (g/mol) M.W. (Observed): 50-60 kDa & 7-9 kDa, reducing conditions; M.W. (theoretical): 43 kDa & 9 kDa
Name Active Heparanase/HPSE
Quantity 5 μg
Source Chinese Hamster Ovary cell line, CHO-derived human Heparanase/HPSE protein Gln36-Ile543 with N-terminal 6-His tag The linker peptide from Ser110-Gln157 is removed by protease treatment
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Symbol HPSE
Conjugate Unconjugated
Recombinant Recombinant
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Heparanase/HPSE
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