R&D Systems™ Recombinant Human Active MAP3K8/Tpl2/COT Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The activity of COT is typically 860-1164 nmol/min/mg using MEK1 and ERK1 substrates and a myelin basic protein (MBP) substrate (see Activity Assay Protocol).

Specifications

• Accession Number: NM_005204
• For Use With (Application): Bioactivity
• Formulation: Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
• Gene ID (Entrez): 1326
• Molecular Weight (g/mol): M.W. (Observed): 70 kDa
• Quantity: 10 μg
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived human MAP3K8/Tpl2/COT protein aa 30-397 Accession # NM_005204
• Storage Requirements: This product is stable at <= -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store (at recommended temperature. Avoid repeated freeze-thaw cycles.)
• Gene Symbol: MAP3K8
• Conjugate: Unconjugated
• Purity or Quality Grade: >70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane)
• Protein: MAP3K8/Tpl2/COT