R&D Systems™ Recombinant Human Active PLK1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The specific activity of PLK1 was equivalent to 42 nmol/min/mg as per Activity Assay Protocol and was equivalent to 17 nmol/min/mg as per radiometric assay.

Specifications

• Accession Number: NM_005030
• For Use With (Application): Bioactivity
• Formulation: Supplied in 50 mM sodium phosphate (pH 7.0), 300 mM NaCl, 0.25 mM DTT, 150 mM imidazole, 0.1 mM PMSF, and 25% glycerol.
• Gene ID (Entrez): 5347
• Molecular Weight (g/mol): M.W. (Observed): 70 kDa
• Quantity: 10 μg
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PLK1 protein
• Storage Requirements: This product is stable at <= -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store (at recommended temperature. Avoid repeated freeze-thaw cycles.)
• Gene Symbol: PLK1
• Conjugate: Unconjugated
• Buffer: Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
• Content And Storage: This product is stable at <= -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store (at recommended temperature. Avoid repeated freeze-thaw cycles.)
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
• Protein: PLK1