R&D Systems™ Recombinant Human Active SGK1 (60-end) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The specific activity of SGK1 was determined to be 99 nmol/min/mg using a Akt (PKB) peptide substrate as per activity assay protocol.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Molecular Weight (g/mol): Observed MW: 73kDa
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived human SGK1 protein aa 60-431 With an N-terminal GST tag
• Storage Requirements: This product is stable at ≤ -70° C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
• Conjugate: Unconjugated
• Content And Storage: This product is stable at <= -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store (at recommended temperature. Avoid repeated freeze-thaw cycles.)
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)