R&D Systems™ Recombinant Human Activin A (CHO derived) Protein

Description

Carrier Free Recombinant Protein

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.200-1.60 ng/mL.

Specifications

• Accession Number: P08476.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 3624
• Molecular Weight (g/mol): MolecularWeight-observed: 13 kDa under reducing conditions and 24 kDa under non-reducing conditions., MolecularWeight-theroretical: 13 kDa
• Quantity: 1 mg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Activin A protein Gly311-Ser426
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: activin AB alpha polypeptide, Activin beta-A chain, erythroid differentiation factor, Erythroid differentiation protein, follicle-stimulating hormone-releasing protein, FSH-releasing protein, inhibin beta A chain, inhibin beta A subunit, Inhibin, beta-1
• Conjugate: Unconjugated
• Reconstitution: Reconstitute 20 μg size at 100 μg/mL in sterile 4 mM HCl. Reconstitute all the other sizes (at 500 μg/mL in sterile 4 mM HCl.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Activin A