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R&D Systems™ Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein

Catalog No. AVI7187050
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AVI7187050 50 μg
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Biotinylated (HEK293-expressed)

Measured by its binding ability in a functional ELISA. When Recombinant Human CD6 Fc Chimera (Catalog # 627-CD ) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag (Catalog # AVI7187) binds with an ED50 of 1.50-12.0 ng/mL.

Specifications

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 214
Molecular Weight (g/mol) M.W.-observed: 100-120 kDa, under reducing conditions., M.W.-theroretical: 84 kDa
Quantity 50 μg
Source Human embryonic kidney cell, HEK293-derived human ALCAM/CD166 protein Human ALCAM (Trp28-Ala526) (C-terminus) Accession # AAB59499.1 DIEGRMD Human IgG1 (Pro100-Lys330) Avi-tag (N-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias activated leucocyte cell adhesion molecule, activated leukocyte cell adhesion moleculeFLJ38514, CD166, CD166 antigen, MEMDMGC71733
Conjugate Biotin
Reconstitution Reconstitute at 250 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein ALCAM/CD166
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