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R&D Systems™ Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Catalog No. AVI11180010
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AVI11180010 10 μg
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His-tag Avi-tag

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human alpha -L-Iduronidase/IDUA His-tag Avi-tag binds to Human alpha-L-Iduronidase/IDUA Antibody (Catalog # AF4119 ) with an ED50 of 0.800-8.00 ng/mL. Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha -L-iduronide. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions.

Specifications

Accession Number P35475.2
For Use With (Application) Bioactivity, Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Gene ID (Entrez) 3425
Molecular Weight (g/mol) M.W.-observed: 81-91 kDa, under reducing conditions, M.W.-theroretical: 73 kDa
Quantity 10 μg
Source Chinese Hamster Ovary cell line, CHO-derived human alpha-L-Iduronidase/IDUA protein Human IDUA (Ala26-Pro653) (N-terminus) Accession # P35475.2 6-His tag Avi-tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias alphaLIduronidase, IDA, IDUA, MPS1, MPSI
Conjugate Biotin
Protein alpha-L-Iduronidase/IDUA
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