R&DSystems™ Recombinant Human Aminopeptidase N/CD13 His Avi Protein, CF

Catalog No.
AVI3815010	10 μg

Catalog No. AVI3815010 Supplier R&DSystems™ Supplier No. AVI3815010

Description

Biotinylated, His-tag, Avi-tag

Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >5000 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human Aminopeptidase N/CD13 His-tag Avi-tag (Catalog # AVI3815) binds Human Aminopeptidase N/CD13 Antibody (Catalog # AF3815 ) with an ED50 of 1.80-18.0 ng/mL.

Specifications

• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in MES and NaCl.
• Gene ID (Entrez): 290
• Molecular Weight (g/mol): M.W.-observed: 116-130 kDa, under reducing conditions., M.W.-theroretical: 105 kD
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Aminopeptidase N/CD13 protein Human APN (Lys69-Lys967) (N-terminus) Accession # AAA51719.1 6-His tag Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, aminopeptidase N, ANPEP, AP-M, APN, AP-N, CD13, CD13 antigen, CD13APN, EC 3.4.11, EC 3.4.11.2, gp150, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, P150, PEPN, PEPNhAPN
• Conjugate: Biotin
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Aminopeptidase N/CD13