R&D Systems™ Recombinant Human ASGR1/ASGPR1 Fc Chimera Protein

Catalog No.	Quantity
10-255-AS050	50 μg

Catalog No. 10-255-AS050 Supplier R&D Systems™ Supplier No. 10255AS050

Description

Measured by its binding ability in a functional ELISA.

• When Recombinant Human ASGR1/ASGPR1 Fc Chimera is immobilized at 2.5 μg/mL (100 μL/well) on a Goat Anti-Human IgG Fc antibody coated plate, the concentration of biotinylated beta-Gal-NAc-PAA that produces 50% of the optimal binding response is 0.75-6 μg/mL.
• Measured by its binding ability in a functional ELISA.
• Immobilized Recombinant Human ASGR1/ASGPR1 Fc Chimera binds human plasma von Willebrand Factor with an ED50 of 0.15 to 0.9 μg/mL.

Specifications

• Accession Number: P07306-1
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 432
• Molecular Weight (g/mol): 59 to 71 kDa, under reducing conditions
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human ASGR1/ASGPR1 protein. MDHuman IgG1 (Pro100-Lys330)IEGRHuman ASGR1 (Asn63-Leu291) Accession # P07306-1N-terminus  C-terminus
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: < 0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS
• Purity or Quality Grade: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining