R&D Systems™ Recombinant Human B3GAT1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q9P2W7
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 27087
• Molecular Weight (g/mol): M.W. (Observed): 50-59 kDa, reducing conditions; M.W. (theoretical): 36 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human beta-1,3-Glucuronyltransferase 1/B3GAT1 protein His25-Ile334, with N-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Beta-1,3-glucuronyltransferase 1, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), CD57, EC 2.4.1.135, galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, GlcAT-P, GLCATP, GLCATPGLCUATP, glcUAT-P, GLCUATP, Glucuronosyltransferase P, HNK1, LEU7, NK1, NK-1, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
• Common Name: beta-1,3-Glucuronyltransferase 1/B3GAT1
• Gene Symbol: B3GAT1
• Biological Activity: Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived His25-Ile334, with N-terminal 6-His tag
• Purity or Quality Grade: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane)
• Protein: beta-1,3-Glucuronyltransferase 1/B3GAT1