R&D Systems™ Recombinant Human B7-1/CD80 Fc Chimera Protein

Description

(High Purity Dimer)

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.4-2 μg/mL in the presence of PHA.

Specifications

• Accession Number: P33681-1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 941
• Molecular Weight (g/mol): M.W. (Observed): 67-85 kDa, reducing conditions; M.W. (theoretical): 50 kDa
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-1/CD80 protein Human B7-1 (Val35-Asn242) (N-terminus) Accession # P33681-1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Specific Reactivity: B7-1/CD80
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-1/CD80