R&D Systems™ Recombinant Human B7-2/CD86 Fc Chimera (CHO-expressed)

Description

A New rh B7-2 protein is Available! It is HEK293 expressed with a His-tag!

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.04-0.2 μg/mL.

Specifications

• Accession Number: AAB03814.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 942
• Molecular Weight (g/mol): M.W. (Observed): 75-100 kDa, reducing conditions; M.W. (theoretical): 52 kDa (monomer)
• Name: B7-2/CD86 Fc Chimera
• Quantity: 100 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human B7-2/CD86 protein Human B7-2/CD86 (Leu20-His239) (N-terminus) Accession # AAB03814.1 Human IgG1 (Pro100-Lys330)(C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD86
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-2/CD86