R&D Systems™ Recombinant Human B7-2/CD86 His-tag Alexa Fluor™ 647 Protein

Catalog No.	Quantity
AFR9090020	20 μg

Catalog No. AFR9090020 Supplier R&D Systems™ Supplier No. AFR9090020

Description

Measured by flow cytometry for its ability to bind anti-human B7-2/CD86 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human B7-2/CD86 His-tag Alexa Fluor™ 647 (Catalog # AFR9090) that produces 50% of the binding response is 6.00-60.0 ng/mL.

Specifications

• Accession Number: NP_787058.4
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 942
• Molecular Weight (g/mol): M.W.-observed: 57-66 kDa, under reducing conditions., M.W.-theroretical: 26 kDa
• Quantity: 20 μg
• Source: Human embryonic kidney cell, HEK293-derived human B7-2/CD86 protein Leu26-Pro247, with a C-terminal 6-His tag
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
• Conjugate: Alexa Fluor 647
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-2/CD86