R&D Systems™ Recombinant Human B7-H2 Fc Chimera Alexa Fluor™ 488 Protein

Description

Measured by flow cytometry for its ability to bind anti-human B7-H2 Antibody conjugated beads. The concentration of Recombinant Human B7-H2 Fc Chimera Alexa Fluor™ 488 (Catalog # AFG165) that produces 50% of the binding response is 20.0-200 ng/mL.

Specifications

• Accession Number: O75144.2
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 23308
• Molecular Weight (g/mol): M.W.-observed: 80-90 kDa, under reducing conditions., M.W.-theroretical: 53.4 kDa (monomer)
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H2 protein Human B7-H2 (Asp19-Ser258) (C-terminus) Accession # O75144.2 DIEGRMD Human IgG1 (Pro100-Lys330) (N-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B7H2, B7H2B7 homolog 2, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50, B7RP-1 LICOS, CD275, CD275 antigen, GL50, GL50transmembrane protein B7-H2 ICOS ligand, ICOSL, ICOS-L, ICOSLG, inducible T-cell co-stimulator ligand, KIAA0653ICOS ligand
• Conjugate: Alexa Fluor 488
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H2/ICOSLG