R&D Systems™ Recombinant Human B7-H2 Fc Chimera Avi-tag Protein

Catalog No.	Quantity
AVI165050	50 μg

Catalog No. AVI165050 Supplier R&D Systems™ Supplier No. AVI165050

Description

Biotinylated

The biotin to protein ratio is 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human ICOS Fc Chimera Protein (Catalog # 169-CS ) is immobilized at 0.5 μg/mL (100 μL/ well), Biotinylated Recombinant Human B7-H2 Fc Chimera Avi-tag (Catalog # AVI165) that produces a 50% optimal binding response is found to be 1-6 ng/mL.

Specifications

• Accession Number: O75144.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 23308
• Molecular Weight (g/mol): M.W.-observed: 80-100 kDa, under reducing conditions, M.W.-theoretical: 55 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human B7-H2 protein Human B7-H2 (Asp19-Ser258) (N-terminus) Accession # O75144.2 DIEGRMD Human IgG1 (Pro100-Lys330) Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B7H2, B7H2B7 homolog 2, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50, B7RP-1 LICOS, CD275, CD275 antigen, GL50, GL50transmembrane protein B7-H2 ICOS ligand, ICOSL, ICOS-L, ICOSLG, inducible T-cell co-stimulator ligand, KIAA0653ICOS ligand
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H2/ICOSLG