R&D Systems™ Recombinant Human B7-H3 Fc Chimera Alexa Fluor™ 647 Protein

Description

Measured by flow cytometry for its ability to bind anti-human B7-H3 Antibody conjugated beads. The concentration of Recombinant Human B7-H3 Fc Chimera Alexa Fluor™ 647 (Catalog # AFR1027) that produces 50% of the binding response is 6.00-60.0 ng/mL.

Specifications

• Accession Number: NP_079516.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Molecular Weight (g/mol): M.W.-observed: 70-80 kDa, under reducing conditions., M.W.-theroretical: 50 kDa (monomer)
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H3 protein Human B7-H3 (Leu29-Pro245) (C-terminus) Accession # NP_079516.1 DIEGRMD Human IgG1 (Pro100-Lys330) (N-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Alexa Fluor 647
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.