R&D Systems™ Recombinant Human B7-H4 Mouse IgG2a Fc Chimera Protein

Description

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.2-4 μg/mL.

Specifications

• Accession Number: NP_078902
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 79679
• Molecular Weight (g/mol): M.W.-observed: 75-85 kDa, under reducing conditions, M.W.-theroretical: 52 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H4 protein Human B7-H4 (Phe29-Ala258) (N-terminus) Accession # NP_078902 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B7h0.5, B7H4, B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1, B7S1VCTN1, B7x, B7XPRO1291, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1, Vtcn1
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H4