R&D Systems™ Recombinant Human BACE-1 Protein

Catalog No.	Quantity
931AS050	50 μg

Catalog No. 931AS050 Supplier R&D Systems™ Supplier No. 931AS050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2 (Catalog # ES004 ). The specific activity is >3.5 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P56817.1
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 23621
• Molecular Weight (g/mol): M.W. (Observed): 68-70 kDa and 65-67 kDa, reducing conditions; M.W. (theoretical): 50 kDa (Pro form) & 47 kDa (Mature form)
• Name: BACE-1
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human BACE-1 protein Thr22-Tyr460 (pro) & Glu46-Tyr460 (mature), both with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Pro and Mature forms
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: BACE1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in sterile, deionized water.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: BACE-1