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R&D Systems™ Recombinant Human BDNF Biotinylated Protein

Catalog No. BT11166025
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25 μg
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BT11166025 25 μg
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Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK ) is immobilized at 0.5μg/mL (100μL/well), Biotinylated Recombinant Human BDNF (Catalog # BT11166) binds with an ED50 of 1.00-10.0 ng/mL.

Specifications

Accession Number P23560.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 627
Molecular Weight (g/mol) M.W. (Observed): 11-16 kDa, under reducing conditions., M.W. (Theoretical): 13.5 kDa
Quantity 25 μg
Source Chinese Hamster Ovary cell line, CHO-derived human BDNF protein His129-Arg247
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias abrineurin, ANON2, brain-derived neurotrophic factor, BULN2, MGC34632, neurotrophin
Conjugate Biotin
Species Human
Reconstitution Reconstitute at 100μg/mL in PBS.
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein BDNF
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