R&D Systems™ Recombinant Human BDNF Protein

Description

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.200-2.00 ng/mL.

Specifications

• Accession Number: P23560.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 627
• Molecular Weight (g/mol): M.W.-observed: 11-15 kDa, under reducing conditions., M.W.-theroretical: 13.5 kDa
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human BDNF protein His129-Arg247
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: abrineurin, ANON2, brain-derived neurotrophic factor, BULN2, MGC34632, neurotrophin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: BDNF