R&D Systems™ Recombinant Human BDNF Protein, Animal-Free

Description

Recombinant Protein

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.100-1.00 ng/mL.

The specific activity of Recombinant Human BDNF is >8.0 x 105 units/mg, which is calibrated against the human BDNF WHO Standard (NIBSC code: 96/534).

Specifications

• Accession Number: P23560.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 627
• Molecular Weight (g/mol): M.W.-observed: 12 kDa, under reducing conditions., M.W.-theroretical: 13.1 kDa
• Quantity: 25 μg
• Source: E. coli-derived human BDNF protein Arg134-Arg247, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: abrineurin, ANON2, brain-derived neurotrophic factor, BULN2, MGC34632, neurotrophin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 25μg size at 250μg/mL in PBS. Reconstitute all other sizes at 500μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
• Protein: BDNF