R&D Systems™ Recombinant Human Betacellulin Protein

Description

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.100-1.50 ng/mL.

Specifications

• Accession Number: P35070.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 685
• Molecular Weight (g/mol): MolecularWeight-observed: 12-15 kDa, under reducing conditions., MolecularWeight-theroretical: 9.9 kDa
• Quantity: 250 μg
• Source: E. coli-derived human Betacellulin/BTC protein Asp32-Gln118
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: betacellulin, BTC, probetacellulin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Betacellulin/BTC