R&D Systems™ Recombinant Human BID (Caspase-8-cleaved) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog # MCTC0 ) to quantify cytochrome c. The typical EC50 for cytochrome c releasing activity is <50 nM. The EC50 for the desired application should be determined. Uncleaved Recombinant Human BID (Catalog # 846-BD ) is available.

Specifications

• Accession Number: P55957
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES-KOH and KCl.
• Gene ID (Entrez): 637
• Molecular Weight (g/mol): M.W. (Observed): 9 kDa &14 kDa, reducing conditions; M.W. (theoretical): 7 kDa (N-terminal fragment) & 15 kDa (C-terminal fragment)
• Name: BID Caspase-8-cleaved
• Quantity: 50 μg
• Source: E. coli-derived human BID protein Met1-Asp195, protein was purified, cleaved by Caspase-8 and further purified
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Gene Symbol: BID
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: BID