R&D Systems™ Recombinant Human BMP-4 Animal-Free Protein

Description

Recombinant Protein

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.

Specifications

• Accession Number: P12644.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Gene ID (Entrez): 652
• Molecular Weight (g/mol): M.W.-observed: 8-9 kDa, under reducing conditions, M.W.-theoretical: 12 kDa
• Quantity: 10 μg
• Source: E. coli-derived human BMP-4 protein Lys303-Arg408 Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: BMP2B, BMP-2 B, BMP2B1, BMP2BMCOPS6, BMP4, BMP-4, Bone morphogenetic protein 2 B, bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, ZYME
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-200 μg/mL in 4 mM HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: BMP-4