R&D Systems™ Recombinant Human BMP-4 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.

Specifications

• Accession Number: Q53XC5
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Gene ID (Entrez): 652
• Molecular Weight (g/mol): M.W. (Observed): 19-24 kDa, reducing conditions 35-41 kDa, non-reducing conditions
• Quantity: 500 μg
• Source: Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied.
• Endotoxin Concentration: <0.01EU per 1μg of the protein by the LAL method.
• Gene Alias: BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, ZYME
• Gene Symbol: BMP4
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 50 to 200μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.