R&D Systems™ Recombinant Human CD155/PVR His-tag Avi-tag Protein

Catalog No.	Quantity
AVI2530050	50 μg
AVI253001M	1 mg

Catalog No. AVI2530050 Supplier R&D Systems™ Supplier No. AVI2530050

Description

Biotinylated

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When 1 μg/mL of Biotinylated Recombinant Human CD155/PVR His-tag Avi-tag protein (Catalog # AVI2530) is immobilized onto Streptavidin coated plate (Catalog # CP004 ), the concentration of Recombinant Human TIGIT Fc Chimera (Catalog # 7898-TGB ) that produces 50% of the optimal binding response is 0.5-3 ng/mL.

Specifications

• Accession Number: NP_006496.4
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 5817
• Molecular Weight (g/mol): M.W. (Observed): 59-67 kDa, under reducing conditions; M.W. (theoretical): 37 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD155/PVR protein Human CD155/PVR (Gly27-Asn343) (N-terminus) Accession # NP_006496.4 HHHHHH Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD155/PVR